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T166N EPHA3 pcDNA3.1
(Plasmid #102742)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102742 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)/myc-His B
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EPHA3
  • Alt name
    MEK4
  • Alt name
    TYRO4
  • Species
    H. sapiens (human)
  • Mutation
    T166N (ACT > AAT)
  • Entrez Gene
    EPHA3 (a.k.a. EK4, ETK, ETK1, HEK, HEK4, TYRO4)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc-6xhis (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note last 10 amino acids in C-terminal are omitted in these constructs. These series of EPHA3 constructs carry missense mutations found in lung and colon cancer.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T166N EPHA3 pcDNA3.1 was a gift from Jin Chen (Addgene plasmid # 102742 ; http://n2t.net/addgene:102742 ; RRID:Addgene_102742)

  • For your References section:

    Effects of cancer-associated EPHA3 mutations on lung cancer. Zhuang G, Song W, Amato K, Hwang Y, Lee K, Boothby M, Ye F, Guo Y, Shyr Y, Lin L, Carbone DP, Brantley-Sieders DM, Chen J. J Natl Cancer Inst. 2012 Aug 8;104(15):1182-97. doi: 10.1093/jnci/djs297. Epub 2012 Jul 24. 10.1093/jnci/djs297 PubMed 22829656
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