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Addgene

Y587E EphA2 pcDNA3
(Plasmid #102730)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102730 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EphA2
  • Alt name
    Eck
  • Species
    M. musculus (mouse)
  • Mutation
    Y587E (TAT > GAG)
  • Entrez Gene
    Epha2 (a.k.a. AW545284, Eck, Myk2, Sek-2, Sek2)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Y587E EphA2 pcDNA3 was a gift from Jin Chen (Addgene plasmid # 102730 ; http://n2t.net/addgene:102730 ; RRID:Addgene_102730)

  • For your References section:

    Identification and functional analysis of phosphorylated tyrosine residues within EphA2 receptor tyrosine kinase. Fang WB, Brantley-Sieders DM, Hwang Y, Ham AJ, Chen J. J Biol Chem. 2008 Jun 6;283(23):16017-26. doi: 10.1074/jbc.M709934200. Epub 2008 Apr 3. 10.1074/jbc.M709934200 PubMed 18387945
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