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Ad-EphA3 KD
(Plasmid #102726)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102726 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAd Easy1
  • Vector type
    Adenoviral
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EphA3
  • Alt name
    Mek4
  • Species
    M. musculus (mouse)
  • Mutation
    Kinase Dead (K657M; AAA > ATG)
  • Entrez Gene
    Epha3 (a.k.a. AW492086, Cek4, EK4, ETK1, End3, Hek, Hek4, Mek4, Tyro4)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct is a adenoviral vector which carries mouse EphA3 cDNA and ready to produce adenovirus which express kinase dead (K657M) EphA3 by transfection into 293A cells followed by PacI digestion

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ad-EphA3 KD was a gift from Jin Chen (Addgene plasmid # 102726 ; http://n2t.net/addgene:102726 ; RRID:Addgene_102726)

  • For your References section:

    Regulation of heart valve morphogenesis by Eph receptor ligand, ephrin-A1. Frieden LA, Townsend TA, Vaught DB, Delaughter DM, Hwang Y, Barnett JV, Chen J. Dev Dyn. 2010 Dec;239(12):3226-34. doi: 10.1002/dvdy.22458. Epub 2010 Oct 19. 10.1002/dvdy.22458 PubMed 20960543
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