Ad-EphA3 WT
(Plasmid
#102725)
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PurposeExpression of WT EphA3
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102725 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAd Easy1
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Vector typeAdenoviral
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Selectable markersEGFP
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEphA3
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Alt nameMek4
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SpeciesM. musculus (mouse)
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Entrez GeneEpha3 (a.k.a. AW492086, Cek4, EK4, ETK1, End3, Hek, Hek4, Mek4, Tyro4)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This construct is a adenoviral vector which carries mouse EphA3 cDNA and ready to produce adenovirus which express wild type EphA3 by transfection into 293A cells followed by PacI digestion
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ad-EphA3 WT was a gift from Jin Chen (Addgene plasmid # 102725 ; http://n2t.net/addgene:102725 ; RRID:Addgene_102725) -
For your References section:
Regulation of heart valve morphogenesis by Eph receptor ligand, ephrin-A1. Frieden LA, Townsend TA, Vaught DB, Delaughter DM, Hwang Y, Barnett JV, Chen J. Dev Dyn. 2010 Dec;239(12):3226-34. doi: 10.1002/dvdy.22458. Epub 2010 Oct 19. 10.1002/dvdy.22458 PubMed 20960543