Ad-EFNA1
(Plasmid
#102724)
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PurposeExpression of EphrinA1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102724 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAd Easy1
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Vector typeAdenoviral
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Selectable markersEGFP
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEFNA1
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Alt nameEphrinA1
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Alt nameB61
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SpeciesH. sapiens (human)
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Entrez GeneEFNA1 (a.k.a. B61, ECKLG, EFL1, EPLG1, GMAN, LERK-1, LERK1, TNFAIP4)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This construct is a adenoviral vector which carries human EFNA1 cDNA and ready to produce adenovirus which express Ephrin-A1 by tranfection into 293A cells followed by PacI digestion.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ad-EFNA1 was a gift from Jin Chen (Addgene plasmid # 102724 ; http://n2t.net/addgene:102724 ; RRID:Addgene_102724) -
For your References section:
Ephrin-A1 facilitates mammary tumor metastasis through an angiogenesis-dependent mechanism mediated by EphA receptor and vascular endothelial growth factor in mice. Brantley-Sieders DM, Fang WB, Hwang Y, Hicks D, Chen J. Cancer Res. 2006 Nov 1;66(21):10315-24. 10.1158/0008-5472.CAN-06-1560 PubMed 17079451