HaloTag-bActinCDS-bActinUTR-MS2V5
(Plasmid #102718)

• Purpose: Halo-Actin reporter for detection of both mRNA and protein
• Depositing Lab: Robert Singer
• Publication: Yoon et al Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):E6877-E6886. Epub 2016 Oct 17.

Backbone

• Vector backbone: pUbC
• Backbone size w/o insert (bp): 6250
• Total vector size (bp): 10067
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HaloTag-bActinCDS-bActin3'UTR-MS2V5
• Species: Synthetic
• Insert Size (bp): 3816
• Promoter: UbC
• Tags / Fusion Proteins:
  • HaloTag (N terminal on insert)
  • MS2V5 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: ClaI (not unique) (unknown if destroyed)
• 5′ sequencing primer: CGC CGA TGA TTA TAT AAG GA (UbC)
• 3′ sequencing primer: cat aaa gag aca gca acc ag (Wpre)

Resource Information

• Supplemental Documents:
  • HaloTag-bActinCDS-bActinUTR-MS2V5.gb
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note, due to the AT rich sequence within the Actin 3'UTR, there are some discrepancies between the Addgene NGS result and the provided full plasmid reference sequence.