hisSUMO-ZFP57
(Plasmid
#102716)
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PurposeExpress DNA binding domain of human Zinc finger protein 57 in E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102716 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneNEB DHFR control plasmid
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Backbone manufacturerNEB
- Total vector size (bp) 3510
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZinc finger protein 57
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Alt nameZFP57
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SpeciesH. sapiens (human)
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Insert Size (bp)912
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Entrez GeneZNF57 (a.k.a. ZNF424)
- Promoter T7
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Tag
/ Fusion Protein
- hisSUMO (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hisSUMO-ZFP57 was a gift from Gary Stormo (Addgene plasmid # 102716 ; http://n2t.net/addgene:102716 ; RRID:Addgene_102716) -
For your References section:
Measuring quantitative effects of methylation on transcription factor-DNA binding affinity. Zuo Z, Roy B, Chang YK, Granas D, Stormo GD. Sci Adv. 2017 Nov 17;3(11):eaao1799. doi: 10.1126/sciadv.aao1799. eCollection 2017 Nov. aao1799 [pii] PubMed 29159284
Map uploaded by the depositor.
