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Addgene

pBabe-puro-Flag-TRAF5
(Plasmid #102679)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102679 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe-puro
  • Backbone size w/o insert (bp) 5086
  • Total vector size (bp) 6883
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TRAF5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1706
  • GenBank ID
    NM_011633
  • Entrez Gene
    Traf5
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site EcoR I (unknown if destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-puro-Flag-TRAF5 was a gift from Hasam Habelhah (Addgene plasmid # 102679 ; http://n2t.net/addgene:102679 ; RRID:Addgene_102679)

  • For your References section:

    TRAF2 suppresses basal IKK activity in resting cells and TNFalpha can activate IKK in TRAF2 and TRAF5 double knockout cells. Zhang L, Blackwell K, Thomas GS, Sun S, Yeh WC, Habelhah H. J Mol Biol. 2009 Jun 12;389(3):495-510. doi: 10.1016/j.jmb.2009.04.054. Epub 2009 May 3. 10.1016/j.jmb.2009.04.054 PubMed 19409903
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