pMIG-Flag-RIPK1-D324A
(Plasmid #102678)

• Purpose: retroviral expression plasmid for RIPK1-D324A
• Depositing Lab: Hasam Habelhah
• Publication: Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38. doi: 10.1128/MCB.00692-15. Epub 2015 Jul 20.

Backbone

• Vector backbone: pMIG-w
• Backbone size w/o insert (bp): 7166
• Total vector size (bp): 9211
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RIPK1
• Species: H. sapiens (human)
• Insert Size (bp): 2045
• Mutation: CHANGE Glutamate 324 to Alanine
• GenBank ID: NM_003804
• Entrez Gene: RIPK1 (a.k.a. AIEFL, IMD57, RIP, RIP-1, RIP1)
• Promoter: unknown
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • IRES-GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II (unknown if destroyed)
• 3′ cloning site: Mfe I/Ecor I (unknown if destroyed)
• 5′ sequencing primer: pLXSN_5

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The RIPK1 insert has a K87Q polymorphism which has no effect on protein activity.