pZH521
(Plasmid
#102674)
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PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102674 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57 (ori changed to p15A)
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Backbone manufacturerGenewiz
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFPmut2
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SpeciesSynthetic
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Insert Size (bp)714
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional Insert: TetR . Please visit https://www.biorxiv.org/content/early/2017/09/23/192963 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZH521 was a gift from Zach Hensel (Addgene plasmid # 102674 ; http://n2t.net/addgene:102674 ; RRID:Addgene_102674) -
For your References section:
A plasmid-based Escherichia coli gene expression system with cell-to-cell variation below the extrinsic noise limit. Hensel Z. PLoS One. 2017 Oct 30;12(10):e0187259. doi: 10.1371/journal.pone.0187259. eCollection 2017. PONE-D-17-11810 [pii] PubMed 29084263