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Addgene

pZH518
(Plasmid #102671)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102671 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57 (ori changed to p15A)
  • Backbone manufacturer
    Genewiz
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GFPmut2
  • Species
    Synthetic
  • Insert Size (bp)
    714

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional Insert: TetR . Please visit https://www.biorxiv.org/content/early/2017/09/23/192963 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZH518 was a gift from Zach Hensel (Addgene plasmid # 102671 ; http://n2t.net/addgene:102671 ; RRID:Addgene_102671)

  • For your References section:

    A plasmid-based Escherichia coli gene expression system with cell-to-cell variation below the extrinsic noise limit. Hensel Z. PLoS One. 2017 Oct 30;12(10):e0187259. doi: 10.1371/journal.pone.0187259. eCollection 2017. PONE-D-17-11810 [pii] PubMed 29084263
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