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Addgene

pcDNA3-neo-Strep_Flag_Nterm
(Plasmid #102644)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102644 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3_neo
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPGGMDYKDDD (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPGGMDYKDDD (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

No insert, but contains a single N-terminus Strepavidin binding peptide and Flag epitopes

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-neo-Strep_Flag_Nterm was a gift from Michael E. Wright (Addgene plasmid # 102644 ; http://n2t.net/addgene:102644 ; RRID:Addgene_102644)

  • For your References section:

    Discovery Proteomics Identifies a Molecular Link between the Coatomer Protein Complex I and Androgen Receptor-dependent Transcription. Hsiao JJ, Smits MM, Ng BH, Lee J, Wright ME. J Biol Chem. 2016 Sep 2;291(36):18818-42. doi: 10.1074/jbc.M116.732313. Epub 2016 Jun 30. 10.1074/jbc.M116.732313 PubMed 27365400
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