p11d-sctRPA
(Plasmid #102614)

• Purpose: Expression of S. cerevisiea RPA in E.coli
• Depositing Lab: Marc Wold
• Publication: Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-506.

Backbone

• Vector backbone: pET11d
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: RPA1
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: RFA1 (a.k.a. YAR007C, BUF2, FUN3, RPA1, RPA70)
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: T7

Gene/Insert 2

• Gene/Insert name: RPA2
• Species: S. cerevisiae (budding yeast)
• Mutation: RFA2 has intron deleted
• Entrez Gene: RFA2 (a.k.a. YNL312W, BUF1, RPA2, RPA32)

Gene/Insert 3

• Gene/Insert name: RPA3
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: RFA3 (a.k.a. YJL173C, RPA14, RPA3)

Resource Information

• Supplemental Documents:
  • 896_p11d-sctRPA_annotated.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

RPA1 5317 RPA2 7292 RPA3 8284 (start of coding region listed)