pcDNA3.1-Hygro(+)-PSTCD-cGAS-delta DBD
(Plasmid
#102606)
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PurposeMammalian expression of mutant cGAS with deletion of DNA binding domain fused to PSTCD tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102606 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1-Hygro(+)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecGAS
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SpeciesH. sapiens (human)
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Mutationdelta K173-I220 and delta H390-C405
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Entrez GeneCGAS (a.k.a. C6orf150, D4, MB21D1, h-cGAS)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
IMPORT NOTES: Special character found in field(Plasmid Name)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-Hygro(+)-PSTCD-cGAS-delta DBD was a gift from Nicolas Manel (Addgene plasmid # 102606 ; http://n2t.net/addgene:102606 ; RRID:Addgene_102606) -
For your References section:
Transmission of innate immune signaling by packaging of cGAMP in viral particles. Gentili M, Kowal J, Tkach M, Satoh T, Lahaye X, Conrad C, Boyron M, Lombard B, Durand S, Kroemer G, Loew D, Dalod M, Thery C, Manel N. Science. 2015 Sep 11;349(6253):1232-6. doi: 10.1126/science.aab3628. Epub 2015 Jul 30. 10.1126/science.aab3628 PubMed 26229115