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Addgene

pcDNA-f:PGC1
(Plasmid #1026)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1026 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 myc-His A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPAR gamma coactivator 1 alpha
  • Alt name
    PGC1 alpha
  • Alt name
    PGC1a
  • Alt name
    PGC-1a
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2400
  • GenBank ID
    NM_008904
  • Entrez Gene
    Ppargc1a (a.k.a. A830037N07Rik, Gm11133, PGC-1, PPARGC-1-alpha, Pgc-1alpha, Pgc1, Pgco1, Ppargc1)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-f:PGC1 was a gift from Bruce Spiegelman (Addgene plasmid # 1026 ; http://n2t.net/addgene:1026 ; RRID:Addgene_1026)

  • For your References section:

    Direct coupling of transcription and mRNA processing through the thermogenic coactivator PGC-1. Monsalve M, Wu Z, Adelmant G, Puigserver P, Fan M, Spiegelman BM. Mol Cell 2000 Aug;6(2):307-16. 10.1016/S1097-2765(00)00031-9 PubMed 10983978
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