pQP-iRIS
(Plasmid #102584)

• Purpose: Regulation of protein localization (near-infrared-blue-light-inducible shuttle)
• Depositing Lab: Vladislav Verkhusha
• Publication: Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038/nchembio.2343.

Backbone

• Vector backbone: pIRES2-EGFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • iRIS.gb
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's provided genbank file. The depositor noted that these discrepancies do NOT affect plasmid function.

New paper providing detailed protocol for using this plasmid- https://www.nature.com/articles/nprot.2018.022