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PurposeLight-controlled activation of expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102583 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSUBCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBphP1-VP16-T2A-NLS-GAL4-QPAS1
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's provided genbank file. The depositor noted that these discrepancies do NOT affect plasmid function.
New paper providing detailed protocol for using this plasmid- https://www.nature.com/articles/nprot.2018.022
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQP-T2A was a gift from Vladislav Verkhusha (Addgene plasmid # 102583 ; http://n2t.net/addgene:102583 ; RRID:Addgene_102583) -
For your References section:
Near-infrared optogenetic pair for protein regulation and spectral multiplexing. Redchuk TA, Omelina ES, Chernov KG, Verkhusha VV. Nat Chem Biol. 2017 Mar 27. doi: 10.1038/nchembio.2343. 10.1038/nchembio.2343 PubMed 28346403
Map uploaded by the depositor.
