HyGCaMP6s
(Plasmid
#102558)
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PurposeExpression of GCaMP6s in Hydra
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102558 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneDerived from pBluescript II SK+
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Backbone manufacturerStratagene
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Vector typeHydra Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGCaMP6s
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SpeciesHydra vulgaris
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Insert Size (bp)1379
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Tag
/ Fusion Protein
- Actin/GCaMP6s fusion protein (C terminal on backbone)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HyGCaMP6s was a gift from Rafael Yuste (Addgene plasmid # 102558 ; http://n2t.net/addgene:102558 ; RRID:Addgene_102558) -
For your References section:
Non-overlapping Neural Networks in Hydra vulgaris. Dupre C, Yuste R. Curr Biol. 2017 Apr 24;27(8):1085-1097. doi: 10.1016/j.cub.2017.02.049. Epub 2017 Mar 30. 10.1016/j.cub.2017.02.049 PubMed 28366745