SRpHi4
(Plasmid
#102544)
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PurposeBacterial expression of EYFP fused to Epidermal Growth Factor with a GST tag for purification
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102544 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-6P-1
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Backbone manufacturerGE Healthcare
- Backbone size w/o insert (bp) 4984
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEYFP-EGF
- Promoter tac
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Coding sequence for human EGF is C-terminal to, and in frame with, EYFP
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SRpHi4 was a gift from Stefan Hell (Addgene plasmid # 102544 ; http://n2t.net/addgene:102544 ; RRID:Addgene_102544) -
For your References section:
SRpHi ratiometric pH biosensors for super-resolution microscopy. Richardson DS, Gregor C, Winter FR, Urban NT, Sahl SJ, Willig KI, Hell SW. Nat Commun. 2017 Sep 18;8(1):577. doi: 10.1038/s41467-017-00606-4. 10.1038/s41467-017-00606-4 [pii] PubMed 28924139
