SRpHi3
(Plasmid
#102543)
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PurposeBacterial expression of sepHluorin fused to the cell penetrating peptide TAT with a GST tag for purification
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102543 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-6P-1
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Backbone manufacturerGE Healthcare
- Backbone size w/o insert (bp) 4984
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTat-sepHluorin
- Promoter tac
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (not destroyed)
- 3′ cloning site Unknown (not destroyed)
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
TAT-peptide in N-terminal to, and in frame with sepHluorin (between GST and sepHluorin); BL-21 cells are used for protein expression
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SRpHi3 was a gift from Stefan Hell (Addgene plasmid # 102543 ; http://n2t.net/addgene:102543 ; RRID:Addgene_102543) -
For your References section:
SRpHi ratiometric pH biosensors for super-resolution microscopy. Richardson DS, Gregor C, Winter FR, Urban NT, Sahl SJ, Willig KI, Hell SW. Nat Commun. 2017 Sep 18;8(1):577. doi: 10.1038/s41467-017-00606-4. 10.1038/s41467-017-00606-4 [pii] PubMed 28924139
