SRpHi1
(Plasmid #102541)

• Purpose: Bacterial expression of EYFP fused to the cell penetrating peptide TAT with a GST tag for purification
• Depositing Lab: Stefan Hell
• Publication: Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 10.1038/s41467-017-00606-4.

Backbone

• Vector backbone: pGEX-6P-1
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 4984
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tat-EYFP
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pGEX5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

TAT-peptide is N-terminal to, and in frame with, EYFP (between GST and EYFP); BL-21 cells are used for protein expression