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PurposeTransgenesis vector based on the Minos transposon, carrying the 3xP3-EGFP marker
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102540 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript
- Total vector size (bp) 5039
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMinos transpoable element with 3xP3-EGFP marker
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMi(3xP3-EGFP) was a gift from Michalis Averof & Charalambos Savakis (Addgene plasmid # 102540 ; http://n2t.net/addgene:102540 ; RRID:Addgene_102540) -
For your References section:
Efficient transformation of the beetle Tribolium castaneum using the Minos transposable element: quantitative and qualitative analysis of genomic integration events. Pavlopoulos A, Berghammer AJ, Averof M, Klingler M. Genetics. 2004 Jun;167(2):737-46. 10.1534/genetics.103.023085 PubMed 15238525