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pcDNA3.1-hPTGS2-2flag
(Plasmid #102498)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102498 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTGS2
  • Alt name
    cyclooxygenase-2
  • Alt name
    COX-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1818
  • GenBank ID
    NM_000963.3
  • Entrez Gene
    PTGS2 (a.k.a. COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag-Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-hPTGS2-2flag was a gift from Jun Yu (Addgene plasmid # 102498 ; http://n2t.net/addgene:102498 ; RRID:Addgene_102498)

  • For your References section:

    Hepatic cyclooxygenase-2 overexpression induced spontaneous hepatocellular carcinoma formation in mice. Chen H, Cai W, Chu ESH, Tang J, Wong CC, Wong SH, Sun W, Liang Q, Fang J, Sun Z, Yu J. Oncogene. 2017 Aug;36(31):4415-4426. doi: 10.1038/onc.2017.73. Epub 2017 Mar 27. 10.1038/onc.2017.73 PubMed 28346420
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