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pSG08-pLac-NRII_H139N
(Plasmid #102465)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102465 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15A
  • Backbone size w/o insert (bp) 2616
  • Total vector size (bp) 3666
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NRII
  • Alt name
    ntrB
  • Insert Size (bp)
    1050
  • Mutation
    Histidine 139 to Asparagine
  • Entrez Gene
    ntrB (a.k.a. PA5124)
  • Promoter pLac

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AAAACCGAATTTTGCTGGGTG
  • 3′ sequencing primer ATGATAAAGAAGACAGTCATAAGTGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2017/04/02/122606 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG08-pLac-NRII_H139N was a gift from Richard Murray (Addgene plasmid # 102465 ; http://n2t.net/addgene:102465 ; RRID:Addgene_102465)

  • For your References section:

    Implementation and System Identification of a Phosphorylation-Based Insulator in a Cell-Free Transcription-Translation System. Guo S, Yeung E, Murray RM. bioRxiv 122606 /10.1101/122606
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