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pSG69-KcsA-Kv1.3-deGFP
(Plasmid #102458)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102458 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBEST
  • Backbone size w/o insert (bp) 2541
  • Total vector size (bp) 3804
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    KL740
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    KcsA-Kv1.3
  • Alt name
    KcsA
  • Alt name
    Kv1.3
  • Species
    Chimera
  • Insert Size (bp)
    495
  • Promoter OR2-OR1-Pr
  • Tag / Fusion Protein
    • His tag (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer AAAACCGAATTTTGCTGGGTG
  • 3′ sequencing primer AACGCCAGTGAAAAGCTC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    deGFP
  • Alt name
    GFP
  • Promoter OR2-OR1-Pr

Cloning Information for Gene/Insert 2

  • Cloning method Unknown
  • 5′ sequencing primer TGCTTGACGATAACCGTC
  • 3′ sequencing primer ATGATAAAGAACACAGTCATAAGTGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2017/01/30/104455 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG69-KcsA-Kv1.3-deGFP was a gift from Richard Murray (Addgene plasmid # 102458 ; http://n2t.net/addgene:102458 ; RRID:Addgene_102458)

  • For your References section:

    Expressing Biologically Active Membrane Proteins in a Cell-Free Transcription-Translation Platform. Guo S, Vaish A, Chen Q, Murray RM. bioRxiv 104455 10.1101/104455
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