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Addgene

pSG13-pBAD-TetRssrA
(Plasmid #102449)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102449 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSC101
  • Backbone size w/o insert (bp) 3878
  • Total vector size (bp) 4499
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TetR
  • Insert Size (bp)
    621
  • Entrez Gene
    tetR (a.k.a. D616_p98057)
  • Promoter pBAD
  • Tag / Fusion Protein
    • ssrA (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AAATGTGCATTGCTGTTCC
  • 3′ sequencing primer AAGACAACATACGACTCTCAAGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2017/04/02/123190 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG13-pBAD-TetRssrA was a gift from Richard Murray (Addgene plasmid # 102449 ; http://n2t.net/addgene:102449 ; RRID:Addgene_102449)

  • For your References section:

    Construction of Incoherent Feedforward Loop Circuits in a Cell-Free System and in Cells. Guo S, Murray RM. ACS Synth Biol. 2019 Mar 15;8(3):606-610. doi: 10.1021/acssynbio.8b00493. Epub 2019 Mar 6. 10.1021/acssynbio.8b00493 PubMed 30790525
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