pSS1 (hLPL-FLAG)
(Plasmid #102447)

• Purpose: Expresses FLAG-tagged human LPL in mammalian cells
• Depositing Lab: Brandon Davies
• Publication: Chi et al J Biol Chem. 2015 May 8;290(19):11865-77. doi: 10.1074/jbc.M114.623769. Epub 2015 Mar 25.

Backbone

• Vector backbone: pcDNA6
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6549
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lipoprotein lipase
• Alt name: LPL
• Species: H. sapiens (human)
• Insert Size (bp): 1560
• Entrez Gene: LPL (a.k.a. HDLCQ11, LIPD)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (C terminal on insert)
  • 6x His (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 promoter forward
• 3′ sequencing primer: BgH polyA reverse

Resource Information

• Supplemental Documents:
  • pSS1 (LPL-His-FLAG).gb
• A portion of this plasmid was derived from a plasmid made by: LPL insert originally cloned by Mark Doolittle (Ben-Zeev O, Mao HZ, Doolittle MH. Maturation of Lipoprotein Lipase in the Endoplasmic Reticulum CONCURRENT FORMATION OF FUNCTIONAL DIMERS AND INACTIVE AGGREGATES. J Biol Chem. 2002 Mar 22;277(12):10727–10738. PMID: 11796709)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry