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pHS2 (hANGPTL4-V5)
(Plasmid #102446)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102446 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA6
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6249
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ANGPTL4
  • Alt name
    FIAF
  • Alt name
    angiopoietin-like 4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1260
  • GenBank ID
    NM_139314
  • Entrez Gene
    ANGPTL4 (a.k.a. ARP4, FIAF, HARP, HFARP, NL2, PGAR, TGQTL, UNQ171, pp1158)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer T7 promoter forward
  • 3′ sequencing primer BgH polyA reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

21bp deletion in the SV40 promoter sequence. May affect blasticidin selection.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHS2 (hANGPTL4-V5) was a gift from Brandon Davies (Addgene plasmid # 102446 ; http://n2t.net/addgene:102446 ; RRID:Addgene_102446)

  • For your References section:

    Angiopoietin-like 4 Modifies the Interactions between Lipoprotein Lipase and Its Endothelial Cell Transporter GPIHBP1. Chi X, Shetty SK, Shows HW, Hjelmaas AJ, Malcolm EK, Davies BS. J Biol Chem. 2015 May 8;290(19):11865-77. doi: 10.1074/jbc.M114.623769. Epub 2015 Mar 25. 10.1074/jbc.M114.623769 PubMed 25809481
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