p2lox-cAMPr R626A
(Plasmid
#102437)
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PurposecAMPr with a point mutation R626A which theoretically decreases its affinity for cAMP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102437 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneP2lox
- Backbone size w/o insert (bp) 3300
- Total vector size (bp) 6000
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Vector typeMammalian Expression, Cre/Lox
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecAMPr
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Insert Size (bp)2268
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MutationPKA-R R626A
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p2lox-cAMPr R626A was a gift from Justin Blau (Addgene plasmid # 102437 ; http://n2t.net/addgene:102437 ; RRID:Addgene_102437) -
For your References section:
cAMPr: A single-wavelength fluorescent sensor for cyclic AMP. Hackley CR, Mazzoni EO, Blau J. Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. doi: 10.1126/scisignal.aah3738. 10.1126/scisignal.aah3738 PubMed 29511120
