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Addgene

pTR47m4-GFP
(Plasmid #102436)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102436 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTR47m4
  • Total vector size (bp) 4328
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For inducible expression of frmR, use in a strain with tetR (e.g. S1030)
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Formaldehyde repressor protein (frmR)
  • Alt name
    frmR
  • Species
    E. coli
  • Insert Size (bp)
    276
  • GenBank ID
    NP_414891.4

Gene/Insert 2

  • Gene/Insert name
    Superfolder GFP
  • Alt name
    sGFP
  • Insert Size (bp)
    717

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTR47m4-GFP was a gift from Gregory Stephanopoulos (Addgene plasmid # 102436 ; http://n2t.net/addgene:102436 ; RRID:Addgene_102436)

  • For your References section:

    Development of a Formaldehyde Biosensor with Application to Synthetic Methylotrophy. Woolston BM, Roth T, Kohale I, Liu D, Stephanopoulos G. Biotechnol Bioeng. 2017 Sep 16. doi: 10.1002/bit.26455. 10.1002/bit.26455 PubMed 28921510
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