pTRE2-Bla(HA–RILP-FLAG)
(Plasmid #102424)

• Purpose: Dual tagged RILP for expression in mammalian cells.
• Depositing Lab: Steven Weinman
• Publication: Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12484-12489. Epub 2016 Oct 17.

Backbone

• Vector backbone: pTre2-Bla
• Backbone size w/o insert (bp): 4858
• Total vector size (bp): 6139
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rab interacting lysosomal protein
• Alt name: RILP
• Species: H. sapiens (human)
• Insert Size (bp): 1281
• GenBank ID: NM_031430.2
• Entrez Gene: RILP (a.k.a. PP10141)
• Promoter: Tet-Responsive
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: pTRE2 5' Sequencing/PCR Primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry