pPB-CAG-rtTA-IRES-Hygro
(Plasmid #102423)

• Purpose: Mammalian expression vector for tetracycline-transactivator (rtTA), IRES-Hygro
• Depositing Lab: Kian Peng Koh
• Publication: Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi: 10.1038/ng.3868. Epub 2017 May 15.

Backbone

• Vector backbone: pPBCAG-rtTAM2-IN
• Backbone manufacturer: Austin Smith lab
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tetracyclin-transactivator (rtTA)
• Promoter: CAG

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: IRES-R

Resource Information

• Supplemental Documents:
  • 102423.gb
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

IRES-Hygro was amplified from pLVX-IRES-Hyg (Clontech) for Gibson cloning using the following primers:

Forward primer: TTTTGACCTTGACATGCTCCCCGGGTAAGCTCGAGACTAGTTCTAGAGCGGCCGCGGATC
Reverse primer: CCATGATATTCGGCAAGCAGGCATCGCCATGGCTATTCCTTTGCCCTCGGACGAGTGCTG