EFR Y877F (17)
(Plasmid
#102415)
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Purposefluorescent tagging. Plant expression of EFR Y877F (17)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102415 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEarleyGate103
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEFR
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SpeciesA. thaliana (mustard weed)
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MutationY877F
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Entrez GeneEFR (a.k.a. AT5G20480, EF-TU receptor, F7C8.70, F7C8_70)
- Promoter 35S
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Tags
/ Fusion Proteins
- mgfp5 (C terminal on backbone)
- 6xHis (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EFR Y877F (17) was a gift from Silke Robatzek (Addgene plasmid # 102415 ; http://n2t.net/addgene:102415 ; RRID:Addgene_102415)