pcDNA3.1-hUCP4-NE
(Plasmid #102362)

• Purpose: Overexpression of solute carrier family 25 member 27 in mammalian cells
• Depositing Lab: Shu Leong Ho
• Publication: Shu Leong Ho Plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5900
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: solute carrier family 25 member 27
• Alt name: UCP4
• Alt name: SLC25A27
• Species: H. sapiens (human)
• Insert Size (bp): 1029
• Entrez Gene: SLC25A27 (a.k.a. UCP4)
• Promoter: CMV
• Tag / Fusion Protein:
  • NE (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: T7 primer/CMV-F primer
• 3′ sequencing primer: BGH-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid encodes a novel 18-amino-acid protein tag - "NE", which can be detected by a specific mouse monoclonal antibody in Western blotting, immunopreciptation, and immunocytochemistry. (Source of antibody: http://www.versitech.hku.hk/reagents/ne/)