Skip to main content
Addgene

pPA016 (delta 86-130 Parkin)
(Plasmid #102357)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102357 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEX-6P1
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 4984
  • Total vector size (bp) 6229
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Parkin
  • Alt name
    PARK2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1275
  • Mutation
    deleted residues 86 to 130
  • Entrez Gene
    Prkn (a.k.a. Park, Park2)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHi (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pGEX Fwd
  • 3′ sequencing primer pGEX Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Parkin is codon-optimized for expression in E. coli.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPA016 (delta 86-130 Parkin) was a gift from Kalle Gehring (Addgene plasmid # 102357 ; http://n2t.net/addgene:102357 ; RRID:Addgene_102357)

  • For your References section:

    A Ubl/ubiquitin switch in the activation of Parkin. Sauve V, Lilov A, Seirafi M, Vranas M, Rasool S, Kozlov G, Sprules T, Wang J, Trempe JF, Gehring K. EMBO J. 2015 Oct 14;34(20):2492-505. doi: 10.15252/embj.201592237. Epub 2015 Aug 7. 10.15252/embj.201592237 PubMed 26254305
Related items:
Commonly requested with: