pLenti TurboRFP BlastR
(Plasmid #102343)

• Purpose: Lentiviral expression of cytoplasmic TurboRFP with Blasticidin selection in cells including neurons
• Depositing Lab: Ghassan Mouneimne
• Publication: Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-0135-18. doi: 10.1523/ENEURO.0135-18.2018. eCollection 2018 Sep-Oct.

Backbone

• Vector backbone: pCIG3 #78264
• Backbone manufacturer: Goodrum Lab, University of Arizona
• Backbone size w/o insert (bp): 7353
• Total vector size (bp): 8046
• Modifications to backbone: Subcloned in Blasticidin resistance gene in place of GFP behind the IRES sequence
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TurboRFP
• Species: Synthetic
• Insert Size (bp): 693
• Promoter: CMV immediate early
• Tag / Fusion Protein:
  • N/A

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: IRES-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry