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Addgene

RNA polymerase beta prime subunit FLAG
(Plasmid #102337)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102337 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RNAP Beta Prime Subunit with C-terminal FLAG TAG
  • Alt name
    Synpcc7942_1524
  • Species
    Synechococcus elongatus PCC7942
  • Tag / Fusion Protein
    • 3x FLAG with 3X GSGS linker (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ania Puszynska

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RNA polymerase beta prime subunit FLAG was a gift from Erin O'Shea (Addgene plasmid # 102337 ; http://n2t.net/addgene:102337 ; RRID:Addgene_102337)

  • For your References section:

    Natural changes in light interact with circadian regulation at promoters to control gene expression in cyanobacteria. Piechura JR, Amarnath K, O'Shea EK. Elife. 2017 Dec 14;6. pii: 32032. doi: 10.7554/eLife.32032. 10.7554/eLife.32032 PubMed 29239721
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