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Addgene

pKDsgRNA-ompT Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pKDsgRNA-ompT with the following enzymes: NotI-HF and NcoI-HF

We predict the following pattern:
Notl-Hf - 6.9 kb
Ncol-HF - 6.9 kb
Notl-HF + NcoI-HF - 5.5kb & 1.4kb

We see the expected result.