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pET-28c(+)-YTHDF3
(Plasmid #102276)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102276 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a(+)
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    YTHDF3
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_152758.5
  • Entrez Gene
    YTHDF3 (a.k.a. DF3)
  • Promoter T7
  • Tag / Fusion Protein
    • 6X HIS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28c(+)-YTHDF3 was a gift from Samie Jaffrey (Addgene plasmid # 102276 ; http://n2t.net/addgene:102276 ; RRID:Addgene_102276)

  • For your References section:

    m6A RNA methylation promotes XIST-mediated transcriptional repression. Patil DP, Chen CK, Pickering BF, Chow A, Jackson C, Guttman M, Jaffrey SR. Nature. 2016 Sep 7;537(7620):369-373. doi: 10.1038/nature19342. 10.1038/nature19342 PubMed 27602518
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