pET-28c(+)-YTHDF2
(Plasmid #102275)

• Purpose: For bacterial expression of His-tagged human YTHDF2
• Depositing Lab: Samie Jaffrey
• Publication: Patil et al Nature. 2016 Sep 7;537(7620):369-373. doi: 10.1038/nature19342.

Backbone

• Vector backbone: pET-28a(+)
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: YTHDF2
• Alt name: YTH N6-methyladenosine RNA binding protein 2
• Alt name: HGRG8
• Species: H. sapiens (human)
• Entrez Gene: YTHDF2 (a.k.a. CAHL, DF2, HGRG8, NY-REN-2)
• Promoter: T7
• Tag / Fusion Protein:
  • 6X HIS (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: TAATACGACTCACTATAGG
• 3′ sequencing primer: GCTAGTTATTGCTCAGCGG

Resource Information

• Supplemental Documents:
  • pET28c(+)-YTHDF2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry