Skip to main content
Addgene

pET-28c(+)-YTHDF2
(Plasmid #102275)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102275 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET-28a(+)
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    YTHDF2
  • Alt name
    YTH N6-methyladenosine RNA binding protein 2
  • Alt name
    HGRG8
  • Species
    H. sapiens (human)
  • Entrez Gene
    YTHDF2 (a.k.a. CAHL, DF2, HGRG8, NY-REN-2)
  • Promoter T7
  • Tag / Fusion Protein
    • 6X HIS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28c(+)-YTHDF2 was a gift from Samie Jaffrey (Addgene plasmid # 102275 ; http://n2t.net/addgene:102275 ; RRID:Addgene_102275)

  • For your References section:

    m6A RNA methylation promotes XIST-mediated transcriptional repression. Patil DP, Chen CK, Pickering BF, Chow A, Jackson C, Guttman M, Jaffrey SR. Nature. 2016 Sep 7;537(7620):369-373. doi: 10.1038/nature19342. 10.1038/nature19342 PubMed 27602518
Related items:
Commonly requested with: