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Addgene

BL21ΔACF
(Bacterial strain #102268)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 102268 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    None
  • Growth Temperature
    30°C
  • Growth Strain(s)
    BL21ΔACF
  • Growth instructions
    Requires low salt (5 g/L NaCl or less) in LB. Strain is intrinsically tetracycline-resistant.
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Genotype = ΔompA ΔompC ΔompF
Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte]

Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BL21ΔACF was a gift from Jack Leo (Addgene plasmid # 102268)

  • For your References section:

    A New Strain Collection for Improved Expression of Outer Membrane Proteins. Meuskens I, Michalik M, Chauhan N, Linke D, Leo JC. Front Cell Infect Microbiol. 2017 Nov 7;7:464. doi: 10.3389/fcimb.2017.00464. eCollection 2017. 10.3389/fcimb.2017.00464 PubMed 29164072
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