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Addgene

pH2B-mPlum/Gag
(Plasmid #102255)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102255 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pvitro1-nmcs
  • Backbone manufacturer
    InvivoGene
  • Backbone size w/o insert (bp) 6295
  • Total vector size (bp) 8126
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Gag

Gene/Insert 2

  • Gene/Insert name
    H2B Plum
  • Insert Size (bp)
    1065

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pH2B-mPlum/Gag was a gift from Suliana Manley (Addgene plasmid # 102255 ; http://n2t.net/addgene:102255 ; RRID:Addgene_102255)
  • For your References section:

    A Quantitative Approach to Evaluate the Impact of Fluorescent Labeling on Membrane-Bound HIV-Gag Assembly by Titration of Unlabeled Proteins. Gunzenhauser J, Wyss R, Manley S. PLoS One. 2014 Dec 10;9(12):e115095. doi: 10.1371/journal.pone.0115095. eCollection 2014. PONE-D-14-41220 [pii] PubMed 25493438