pH2B-mPlum/Gag
(Plasmid
#102255)
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Purposeco-expression of Gag and H2B Plum in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102255 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepvitro1-nmcs
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Backbone manufacturerInvivoGene
- Backbone size w/o insert (bp) 6295
- Total vector size (bp) 8126
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameGag
Gene/Insert 2
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Gene/Insert nameH2B Plum
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Insert Size (bp)1065
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pH2B-mPlum/Gag was a gift from Suliana Manley (Addgene plasmid # 102255 ; http://n2t.net/addgene:102255 ; RRID:Addgene_102255) -
For your References section:
A Quantitative Approach to Evaluate the Impact of Fluorescent Labeling on Membrane-Bound HIV-Gag Assembly by Titration of Unlabeled Proteins. Gunzenhauser J, Wyss R, Manley S. PLoS One. 2014 Dec 10;9(12):e115095. doi: 10.1371/journal.pone.0115095. eCollection 2014. PONE-D-14-41220 [pii] PubMed 25493438