Kif5A-GFP-CIBN
(Plasmid #102252)

• Purpose: Expresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)
• Depositing Lab: Bianxiao Cui
• Publication: Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi: 10.1016/j.chembiol.2015.04.014. Epub 2015 May 9.

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 3800
• Total vector size (bp): 6800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Kif5A-GFP-CIB1
• Alt name: Kif5A
• Alt name: CIB1
• Alt name: CIBN
• Species: R. norvegicus (rat), A. thaliana (mustard weed)
• Insert Size (bp): 3000
• Entrez Gene: Kif5a
• Entrez Gene: CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CMV forward

Resource Information

• Supplemental Documents:
  • pEGFP_ Kif5A-GFP-CIBN.ape
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry