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PurposeExpresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 7400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLAMP-mCherry-CRY2
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Alt nameLAMP1
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Alt nameCRY2
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SpeciesH. sapiens (human), A. thaliana (mustard weed)
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Insert Size (bp)3500
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Entrez GeneLAMP1 (a.k.a. CD107a, LAMPA, LGP120)
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Entrez GeneCRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LAMP-mCherry-CRY2 was a gift from Bianxiao Cui (Addgene plasmid # 102249 ; http://n2t.net/addgene:102249 ; RRID:Addgene_102249) -
For your References section:
Optogenetic control of molecular motors and organelle distributions in cells. Duan L, Che D, Zhang K, Ong Q, Guo S, Cui B. Chem Biol. 2015 May 21;22(5):671-82. doi: 10.1016/j.chembiol.2015.04.014. Epub 2015 May 9. 10.1016/j.chembiol.2015.04.014 PubMed 25963241