CRY2-mCherry-Miro1TM
(Plasmid #102247)

• Purpose: Expresses fusion of CRY2PHR with mCherry and transmembrane domain of Miro1
• Depositing Lab: Bianxiao Cui
• Publication: Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi: 10.1016/j.chembiol.2015.04.014. Epub 2015 May 9.

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 6400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CRY2PHR-mCherry-Miro1TM
• Alt name: CRY2
• Alt name: Miro1
• Alt name: RHOT1
• Species: H. sapiens (human), A. thaliana (mustard weed)
• Insert Size (bp): 2400
• Entrez Gene: RHOT1 (a.k.a. ARHT1, MIRO-1, MIRO1)
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV forward

Resource Information

• Supplemental Documents:
  • CRY2PHR-mcherry-Miro1TM_real.str
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry