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Addgene

CRY2 mCherry Sec61B
(Plasmid #102246)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102246 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CRY2PHR-mCherry-SEC61beta
  • Alt name
    CRY2-mCherry-SEC
  • Alt name
    CRY2
  • Alt name
    SEC61
  • Species
    H. sapiens (human), A. thaliana (mustard weed)
  • Insert Size (bp)
    2500
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CRY2 mCherry Sec61B was a gift from Bianxiao Cui (Addgene plasmid # 102246 ; http://n2t.net/addgene:102246 ; RRID:Addgene_102246)
  • For your References section:

    The Dual Characteristics of Light-Induced Cryptochrome 2, Homo-oligomerization and Heterodimerization, for Optogenetic Manipulation in Mammalian Cells. Che DL, Duan L, Zhang K, Cui B. ACS Synth Biol. 2015 Oct 16;4(10):1124-35. doi: 10.1021/acssynbio.5b00048. Epub 2015 Jun 8. 10.1021/acssynbio.5b00048 PubMed 25985220