CRY2 mCherry Sec61B
(Plasmid
#102246)
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PurposeExpresses fusion of CRY2PHR with mCherry and Sec61B
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102246 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 6500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCRY2PHR-mCherry-SEC61beta
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Alt nameCRY2-mCherry-SEC
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Alt nameCRY2
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Alt nameSEC61
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SpeciesH. sapiens (human), A. thaliana (mustard weed)
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Insert Size (bp)2500
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Entrez GeneCRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ATGGGCGGTAGGCGTGTA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CRY2 mCherry Sec61B was a gift from Bianxiao Cui (Addgene plasmid # 102246 ; http://n2t.net/addgene:102246 ; RRID:Addgene_102246) -
For your References section:
The Dual Characteristics of Light-Induced Cryptochrome 2, Homo-oligomerization and Heterodimerization, for Optogenetic Manipulation in Mammalian Cells. Che DL, Duan L, Zhang K, Cui B. ACS Synth Biol. 2015 Oct 16;4(10):1124-35. doi: 10.1021/acssynbio.5b00048. Epub 2015 Jun 8. 10.1021/acssynbio.5b00048 PubMed 25985220