pHR-EF1a-dCas9-HA-BFP-KRAB-NLS
(Plasmid #102244)

• Purpose: Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter
• Depositing Lab: Jacob Corn
• Publication: Corn Lab Cas9 plasmids (unpublished)

Backbone

• Vector backbone: pHR
• Backbone size w/o insert (bp): 9651
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: TagBFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: dCas9-HA-BFP-KRAB-NLS
• Species: H. sapiens (human)
• Insert Size (bp): 5169
• Promoter: EF1a
• Tag / Fusion Protein:
  • HA-TagBFP-KRAB-NLS (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • IGI-P0220-pHR-EF1a-dCas9-HA-BFP-KRAB-NLS.gb
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains HA tag and TagBFP fusion. The plasmid is derived from Addgene Plasmid #46911. The promoter was changed to human EF1a and the NLS was moved to the C-terminus of the fusion protein.