pFastBac_Dual_SNF2L-opt_p48
(Plasmid #102243)

• Purpose: Expression of human codon optimized human SNF2L and pRbAp48 from pFastBac dual
• Depositing Lab: Joe Landry
• Publication: pFastBac1_FLAG-BPTF (unpublished)

Backbone

• Vector backbone: pFastBac Dual
• Backbone manufacturer: Invitrogen
• Total vector size (bp): 9605
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: SNF2L
• Species: H. sapiens (human)
• Insert Size (bp): 3177
• Mutation: codon optimized for expression
• Entrez Gene: SMARCA1 (a.k.a. ISWI, NURF140, SNF2L, SNF2L1, SNF2LB, SNF2LT, SWI, SWI2, hSNF2L)
• Promoter: p10

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sma1 (not destroyed)
• 3′ cloning site: Kpn1 (not destroyed)
• 5′ sequencing primer: None
• 3′ sequencing primer: TK-pA-R

Gene/Insert 2

• Gene/Insert name: pRbAp48
• Alt name: RBBP4
• Species: H. sapiens (human)
• Insert Size (bp): 1278
• GenBank ID: NM_005610.2
• Entrez Gene: RBBP4 (a.k.a. NURF55, RBAP48, lin-53)
• Promoter: Polyhedrin

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hpa1 (destroyed during cloning)
• 3′ cloning site: Sph1 (not destroyed)
• 5′ sequencing primer: Polyhedrin-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA