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Addgene

pFastBac1_FLAG-BPTF
(Plasmid #102242)

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Item Catalog # Description Quantity Price (USD)
Plasmid 102242 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4775
  • Total vector size (bp) 13289
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flag-BPTF
  • Alt name
    FALZ
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8427
  • Entrez Gene
    BPTF (a.k.a. FAC1, FALZ, NEDDFL, NURF301)
  • Promoter Polyhedron
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site Nhe1 (not destroyed)
  • 5′ sequencing primer Unknown
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BPTF insert contains amino acid residues 140-2903 with C1746R, T1752A, D1852N variants compared to the NCBI reference [NM_004459.6].

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac1_FLAG-BPTF was a gift from Joe Landry (Addgene plasmid # 102242 ; http://n2t.net/addgene:102242 ; RRID:Addgene_102242)
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