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Addgene

pMZ0002
(Plasmid #102239)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102239 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPROEX1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing laboratory recommends using strain BL21(DE3) for protein expression
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    N-ethylmaleimide sensitive factor
  • Alt name
    NSF
  • Species
    Chinese hamster
  • Tag / Fusion Protein
    • 6x His tag (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMZ0002 was a gift from Axel Brunger (Addgene plasmid # 102239 ; http://n2t.net/addgene:102239 ; RRID:Addgene_102239)

  • For your References section:

    Mechanistic insights into the recycling machine of the SNARE complex. Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT. Nature. 2015 Feb 5;518(7537):61-7. doi: 10.1038/nature14148. Epub 2015 Jan 12. 10.1038/nature14148 PubMed 25581794
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